Journal: Scientific Reports

Article Title: Potentials of the elevated circulating miR-185 level as a biomarker for early diagnosis of HBV-related liver fibrosis

doi: 10.1038/srep34157

Figure Lengend Snippet: ( A ) Differentially-changed blood miRNA levels in DMN liver fibrosis rat model. The miRNA levels were determined using microRNA array and validated by qRT-PCR. ( B ) Blood miR-185 levels in normal rats (NC, n = 6), rats with DMN-induced early-stage liver fibrosis (n = 10), and rats with advanced-stage liver fibrosis (n = 11). The line indicates the median value in each group. Fold-changes were calculated using the 2 -ΔΔCt method. The Mann-Whitney test was used as the statistical significance test. ( C ) Histological confirmation of rat liver fibrosis induced by DMN. ( D ) Blood miR-185 levels in normal rats (NC, n = 6), rats with BDL-induced early-stage liver fibrosis (n = 8), and rats with late-stage liver fibrosis (n = 11). The line indicates the median value in each group. Fold-changes were calculated using the 2 −ΔΔCt method. The Mann-Whitney test was used as the statistical significance test. ( E ) Liver fibrosis staging in rats subjected to BDL. HE: HE staining; masson: masson staining; VG: VG staining. The pictures were taken using a microscope (200x). ( A ) control group (F0); ( B ) stage 1 fibrosis (F1); ( C ) stage 2 fibrosis (F2); D, stage 3 fibrosis (F3); E, stage 4 fibrosis(F4). Representative data from three repeats are shown.

Article Snippet: To examine the potential involvement of microRNAs in the initiation and progression of liver fibrosis, a miRNA expression profile of 12 blood RNA samples was generated using a microRNA microarray assay on the ParafloTM platform (LC Sciences) and Sanger miRBase 18.0. ( ).

Techniques: Quantitative RT-PCR, MANN-WHITNEY, Staining, Microscopy, Control

Journal: Genes & Diseases

Article Title: Recent advances in elucidating the genetics of common variable immunodeficiency

doi: 10.1016/j.gendis.2019.10.002

Figure Lengend Snippet: Advantages and limitations of different genomic, transcriptomic and proteomics based platforms in Common variable immunodeficiency disease. ELISA, Enzyme-linked immunosorbent assay; SNP, Single nucleotide polymorphism.

Article Snippet: The major breakthrough in the sequencing technology, came with 13-years long, Human Genome Project (HGP) which was completed in 2003. , With the transition of sequencing technology to development of next generation sequencing based technology (Illumina, Roche 454, Ion Torrent/PGM sequencing, Oxford Nanopore) for rapid amplification of larger stretch of human genome, the sequencing platforms allowed for sequencing with 30× coverage or more.

Techniques: Enzyme-linked Immunosorbent Assay, Genome Wide, Biomarker Discovery, Sequencing, Electrophoresis, Next-Generation Sequencing, Amplification, High Throughput Screening Assay, Gene Expression, Generated, Microarray, Hybridization, Sample Prep, Transcriptome Wide Gene Expression, DNA Methylation Assay, Methylation, Quantitative Proteomics, Fourier Transform Infrared Spectroscopy, Spectroscopy